We isolated colonic adenomas, and after exclusion of immune, endothelial, erythroid, and epithelial cells, using the lineage negative (Lin−) markers CD45, CD31, Ter119, and EpCAM, respectively, we isolated Col6a1+/GFP+ and Col6a1−/GFP− (or Tomato+) CAFs by FACS sorting and performed 3′ mRNA sequencing (Figure 1A,B). Here, TBX1 is linked to colon adenoma.