Additionally, the effect of shionone on the STAT3 and MEK/ERK signaling pathways was also studied by Western blot analysis, and the phosphorylation of STAT3, MEK, and ERK was found to be decreased in dose–dependent manners, which clearly indicates that these may be the target pathways for the anti-breast cancer activity of shionone [34]. This evidence concerns the gene STAT3 and breast cancer.