Two patients with an initial decrease in PSA level after salvage radiotherapy or the radiotherapy of lymph node metastases showed the same PSA level as was shown at the time of the [89Zr]Zr-PSMA-DFO PET/CT after 32 months and 42 months, respectively. This evidence concerns the gene FOLH1 and metastatic malignant neoplasm in the lymph nodes.