To study the antioxidant and anti-inflammatory effects of the DHCA, DHFA, and HHA coffee metabolites in the 3T3-L1 fibroblast cell line, a cell culture model of TNF-α-stimulated cells, similar to that previously used in hepatoma HepG2 cells [23] and endothelial EA.hy926 cells [28], was adapted to cultured 3T3-L1 cells. This evidence concerns the gene TNF and hepatocellular carcinoma.