Serum S100B and osteopontin levels were significantly higher in the metastatic group (p < 0.001 and p = 0.002, respectively); however, serum LDH levels showed no significant difference (p = 0.107) between the non-metastatic and metastatic melanoma patient groups (Table 1, Figure 1). This evidence concerns the gene SPP1 and metastatic melanoma.