Densitometry results of Western blot analysis showed a decrease in DNMT3B, DNMT1, and MBD2 in prostate cancer cells upon treatment with saffron compared to untreated cells (Figure 4E), suggesting a global decrease in DNA methylation which might be associated with increased specific genes in prostate cells (increased tumor-specific gene expression, due to decreased methylase levels). This evidence concerns the gene DNMT1 and prostate carcinoma.