However, OLE-JGF feeding did not alter the expression of AMPK phosphorylation and total AMPK in the liver of HFD-fed mice, which was inconsistently with attenuated SREBP-1c expression, indicating that the inhibition of hepatic lipogenesis and anti-obesity effects of OLE-JGF in HFD-fed mice may not be required for the involvement of AMPK signaling pathway. The gene discussed is SREBF1; the disease is obesity due to melanocortin 4 receptor deficiency.