Over and above in silico tools and pathway analysis, we demonstrate the functional presence of an miR-494-5p physical binding site within the 3′UTR of JAK1 and show that miR-494-5p overexpression in human CRC cells significantly inhibits JAK1 3′UTR activity and JAK 1 expression at the mRNA and protein level. Here, JAK1 is linked to colorectal carcinoma.