ROS1 and glioblastoma: C’-terminal FLAG-tagged wild-type ROS1 and FLAG-tagged KLC1-ROS1 were stably expressed in A172 GBM cells, as shown in Figure 1, and immunoblot analysis of fractionated cell lysates (the lysates of plasma membrane, total cytosol, and nucleus) of these cells demonstrated that KLC1-ROS1 fusion was specifically localized in cytosol, while wild-type ROS1 was localized in both the plasma membrane and cytoplasm, but was rather enriched in cytoplasm.