Therefore, to investigate the oncogenic roles of KLC1-ROS1 fusion in glioma cells, plasmids encoding C’-terminal FLAG-tagged wild-type ROS1 and KLC1-ROS1 fusion as well as the empty vector were stably expressed in the A172 GBM cell line, which did not express endogenous wild-type ROS1 [12] and has already been used as the manipulable model cell in our previous study regarding KLC1-ROS1 fusion [12]. This evidence concerns the gene ROS1 and central nervous system cancer.