A total of 4626 DEGs, 6129 DEGs, and 2598 DEGs were found in liver, spleen, and LDM tissues, respectively, with the DEGs in the liver mainly enriched in non-alcoholic fatty liver disease, endocytosis, and the p53 signaling pathway, indicating a difference in fatty metabolism and immune response between WZS and LW pigs (Figure 5A; Supplementary Table S9). This evidence concerns the gene TP53 and metabolic dysfunction-associated steatotic liver disease.