In the third level metabolic pathway, KO00642 (ethylbenzene degradation), KO03320 (PPAR signaling pathway), KO00965 (betalain biosynthesis), KO05322 (systemic lupus erythematosus), and KO05131 (shigellosis) were significantly up-regulated in semi-free-range cranes. This evidence concerns the gene PPARA and systemic lupus erythematosus.