Scratch and Transwell assays demonstrated a notable decrease in migration of 231 and MCF7 cells upon CENPM knockdown (Fig. 14B-D).Quantitative and statistical analysis was performed on the results of the colony formation assay, transwell assay, and scratch assay (Fig. 14E-G).To obtain a more comprehensive understanding of how CENPM affects the behavior of breast cancer cells and their immune infiltration, we conducted an experiment where M0 macrophages were cultured with supernatants from breast cancer cells in both the CENPM knockdown and NC groups. This evidence concerns the gene CENPM and breast cancer.