To test whether MST4 participates in microglial activation in ARS, we conducted immunofluorescence staining of MST4 in the brain and found that it was widely distributed in several regions, including the accumbens nucleus, caudate putamen, paraventricular hypothalamic nucleus, and CeA (Supplementary Fig. 13, and Fig. 5a). The gene discussed is STK26; the disease is Axenfeld-Rieger syndrome.