Further, in a migraine model, applying C48/80 and PACAP 1-38 directly onto the dura increased mechanical facial hypersensitivity which was significantly reduced in Mrgprib2−/− mice.79 To test the role of MRGPRX2 specifically in mast cells in vivo, transgenic mice harboring MRGPRX2 (ROSA26lsl-MRGPRX2) were crossed with Mrgprb2Cre mice, generating Mrgprb2MRGPRX2 animals. Here, MRGPRX2 is linked to migraine disorder.