PROS1 and infection: Additionally, proteins associated with virulence were diminished, including Prepilin leader peptidase PilD and assembly protein PA3688, virulence regulator PvrA [21], pyocin F2, T3SS export proteins PscD and PscG, and the Type VI secretion system (T6SS) baseplate component TssK1 [22] (Fig 1H), consistent with the results from infection models.