To verify the role of DDX1 as a passenger in the pathogenesis of neuroblastoma in an in vivo experimental system, we generated a transgenic zebrafish line that stably expresses human DDX1 in the peripheral sympathetic nervous system and compared those to zebrafish expressing both DDX1 and MYCN (Supplementary Fig. S4A–S4C). Here, MYCN is linked to neuroblastoma.