Literature has also shown high p16 sensitivity (94%) and low specificity (82%) as compared to PCR/ISH mainly due to subjective interpretation associated with weak/equivocal staining resulting in high amounts of false-positive cases which makes ICC technique unreliable in these situations.23,24 Pathak et al.25 determined HPV status of the tumors using HPV-DNA PCR and p16 immunostaining in oral dysplasia and oral carcinoma in 50 patients, out of which 83% HPV DNA positive cases by conventional PCR were also positive for p16 expression. Here, CDKN2A is linked to intrahepatic cholangiocarcinoma.