Moreover, the protein expression profiles in the natural killer cell-mediated cytotoxicity pathway also hinted at the reduced activity of hepatic NK cells in the NASH-FMT group, including downregulated NKG2AB, NKGP46, NKG2DL, IFN-γ, granzyme, TNF-α, and perforin (Supplementary Figure S6). Here, PRF1 is linked to metabolic dysfunction-associated steatohepatitis.