Compared with that in the NCD group, the focal adhesion pathway and TNF signaling pathway were significantly altered, and the NAFLD pathway was significantly upregulated in the NASH-FMT group and was statistically reversed by B. uniformis and B. bifidum (not in the top 20 pathways) transplantation (Supplementary Figure S5). The gene discussed is TNF; the disease is metabolic dysfunction-associated steatotic liver disease.