To further validate the interaction between NUDT21 and NDUFS2, IF assay was performed on pancreatic cancer and adjacent tissues obtained from surgery, and the results showed that NUDT21 and NDUFS2 were co-localized in pancreatic cancer and they were both highly expressed in pancreatic cancer than adjacent tissues (Fig. 7H). This evidence concerns the gene NUDT21 and familial pancreatic carcinoma.