Eosinophils from Enpp2-knockdown tumors displayed irregularly organized membrane architecture, multilobed segmented nuclei, empty vacuoles within the cytoplasm and less intense staining of cytosolic granules (Fig. 3b), potentially indicating eosinophil activation and degranulation in the Enpp2-knockdown context, consistent with our tumor tissue TEM analysis. This evidence concerns the gene ENPP2 and neoplasm.