Moreover, in synovial fluid macrophages from patients with gout and calcium pyrophosphate deposition (CPPD), both of which are NLRP3 inflammasome-dependent diseases [30, 43], NCOA6 was detected in cytoplasmic specks (single and multiple) that colocalized with ASC, as determined by immunofluorescence staining (Fig. 6E, F), which is quite similar to the findings observed in PMA-primed THP-1 cells (Fig. 2E, F). Here, PYCARD is linked to glycogen storage disease VI.