In order to study the role of mitofusin 1 in protein secretion in senescence we worked with a stable cell line obtained by transducing mouse B16-F1 melanoma cells with lentiviral particles carrying shRNA targeting Mfn1 (shMfn1), or a scrambled sequence (shScr), and selected with puromycin10. This evidence concerns the gene MFN1 and melanoma.