Of note, the abundance of anti-spike S1 IgM from the pooled severe COVID-19 cohort was 1.7-fold higher than the nonsevere cohort as indicated by LC-MS/MS analysis of the Coomassie-stained Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of spike S1-specific immunoglobulins (Supplemental Figs. 6c and d). The gene discussed is CD40LG; the disease is COVID-19.