Our experiments further shed light on the relationship between local RAS and PCOS, and trehalose may act through local RAS.Although the activation/inhibition of specific molecules was not used for causal validation in this study, OVRAS are associated with oxidative stress and apoptosis in other diseases, and we will further investigate by targeting ATR1,ATR2 and MASR in the future. The gene discussed is AGTR1; the disease is polycystic ovary syndrome.