We hypothesized that an increase in METRNL due to muscle contraction in line with intracellular calcium elevation increased the phosphorylation of TBC1D1 (Ser237) but inhibited TBC1D4 (Thr642) phosphorylation, indicating that METRNL stimulates the transportation and movement of GLUT4, specifically via AMPK-TBC1D1, so contribute to the improvement and treatment of insulin resistance [7]. Here, TBC1D1 is linked to Insulin resistance.