The anti-CAIX G36 CAR CD28 or BBζ peripheral blood mononuclear cells (PBMCs) secreting anti-PD-L1 IgG4 were recently tested in low doses in a similar orthotopic ccRCC model, equivalent to ≅ 107 CAR-T PBMCs/kg, and the CD28-based construction was superior to BBζ when immune checkpoint blockade via PD-L1 was used in combination. This evidence concerns the gene CD28 and nonpapillary renal cell carcinoma.