Among the top 20 downregulated DEGs (Fig. 2C), we highlight ZW10 Interacting Kinetochore Protein (ZWINT), Establishment Of Sister Chromatid Cohesion N-Acetyltransferase 2 (ESCO2), Minichromosome Maintenance Complex Component 6 (MCM6), progesterone receptor (PGR) and Minichromosome Maintenance Complex Component 4 (MCM4) (log2FC = -2.57, -2.53, -2.48, -2.21, and − 2.07, respectively) based on their possible associations with recurrent pregnancy loss (RPL) and preeclampsia. This evidence concerns the gene ESCO2 and preeclampsia.