To explore the effect of PCYT1B on tumorigenesis, we established an HCC model (20), in which p53fl/fl;alb-cre mice fed a choline-deficient diet were coinjected with the MYC oncogene along with PT3 plasmid DNA expressing PCYT1B or GFP control (Figure 5C). This evidence concerns the gene MYC and hepatocellular carcinoma.