PIK3CA and neoplasm: We first performed RNA-Seq on isogenic DLD1 PIK3CA WT-only and Mut-only cell lines (14) treated with either vehicle or the combination of CB-839 and 5-FU, because the drug combination induced more NETs and tumor-infiltrating neutrophils in PIK3CA-Mut–only tumors than the isogenic WT-only tumors (Supplemental Figure 3, A and B).