This study compared 25 bodybuilders with gingivitis who used protein supplements (BB-G) to 25 non-exercising males with (G) and 25 without gingivitis (H). It analyzed saliva, gingival fluid, and serum for gene expression of IL-1β, ASC, and CASP1. Periodontal health was assessed through various indices including plaque index (PI), gingival index (GI), and bleeding on probing (BOP). The gene discussed is CASP1; the disease is gingivitis.