To detect CAF subpopulations, we performed multiplex immunofluorescence to detect markers of hematopoietic cells (CD45), epithelial cells (pan-cytokeratin [CK]), and fibroblasts (α-smooth muscle actin [α-SMA], fibroblast activation protein (FAP), fibroblast specific protein 1 [FSP-1], and Thy-1) on surgically resected invasive lobular carcinoma of the breast (Figure 2). Here, ACTA1 is linked to neoplasm.