Quantitative immunoblotting showed highly variable levels of total LRRK2 which were not significantly different amongst control, R1441G-LRRK2 PD, R1441G-LRRK2 NMC, G2019S-LRRK2 PD, G2019S-LRRK2 NMC and idiopathic PD LCLs, whilst the levels of total Rab10 were more similar (Fig. 5a, b). This evidence concerns the gene LRRK2 and Parkinson disease.