A single subretinal injection of v3 PE-eVLPs demonstrated efficient in vivo prime editing in mouse models, correcting a 4-bp deletion in Mfrp in the rd6 mouse model of retinal degeneration (15% average efficiency) and correcting an Rpe65 substitution to partially rescue visual function in the rd12 model (7.2% average efficiency). The gene discussed is MFRP; the disease is retinal degeneration.