When we transduced primary mouse embryonic neurons with an AAV encoding hnRNP A1(F263S), we found that the mutant dysfunctional hnRNP A1 caused increased accumulation of Pkm2 (Supplementary Fig. 8e), thus directly confirming that neuronal hnRNP A1 dysfunction leads to the changes in PKM/Pkm splicing we observed in MS and EAE. This evidence concerns the gene PKM and myeloid sarcoma.