The β-catenin staining technique is used to assess the level of β-catenin protein expression, which is considered an important regulatory factor to cellular proliferation and aids in T-suppressor cells (aiding in cellular proliferations).The present study showed reduced Bax protein expression and increased β-catenin protein expression in cancer control rats, indicating a significant imbalance between these two proteins could lead to cellular dysfunctionality and changes in the mitochondrial route of apoptosis53,54. This evidence concerns the gene BAX and cancer.