Based on the good fit between the model and the map as well as our MS analysis (Supplementary Table 7), we placed one copy of adenylate kinase 8 (AK8), a nucleoside monophosphate kinase associated with congenital hydrocephalus in knockout mice51, in the stalk region of RS1 together with five copies of Rsph22 (Fig. 3d). The gene discussed is DYNLL2; the disease is congenital hydrocephalus.