Based on the good fit between the model and the map as well as our MS analysis (Supplementary Table 7), we placed one copy of adenylate kinase 8 (AK8), a nucleoside monophosphate kinase associated with congenital hydrocephalus in knockout mice51, in the stalk region of RS1 together with five copies of Rsph22 (Fig. 3d). This evidence concerns the gene AK8 and congenital hydrocephalus.