We then tested the methylation level of cg04255978, the most significantly methylated CpG site in NEURL3 gene, in another 8 NPC and 8 normal nasopharyngeal tissues by pyrophosphate sequencing, and found that the methylation level of cg04255978 was significantly increased in NPC tissue samples (Fig. 1e-f). The gene discussed is NEURL3; the disease is nasopharyngeal carcinoma.