Analyses using scRNA-seq profiles of human PCa bone metastasis revealed transcriptional patterns similar to those present in the mesenchymal clusters from the PRN model (c5-c7), comprising more than 60% of bone stromal cells (Fig. 7c) and exhibiting a high expression of POSTN (Fig. 7d), together with osteoblasts marker genes, such as BGN, RUNX2, and SPP1 (Fig. 6c, d). This evidence concerns the gene SPP1 and posterior cortical atrophy.