Interestingly, in human neuroblastoma SHSY5Y cells, treatment with different doses of the pro-inflammatory factor TNF-α (1, 5, 10 ng/ml) significantly increased the level of intracellular CARS protein and induced secretion of CARS protein at 24 h (Additional file 3: Fig. S19a, b, e, f). The gene discussed is TNF; the disease is neuroblastoma.