No significant difference was detected in the total distance moved (OFT), time and entry in the central area (OFT), time and entry in the open arms (EPM), or the latency to fall (rotarod), revealing that overexpression of CARS in the hippocampal neurons of the C57BL/6J mice had no effect on the anxiety level or locomotor activity. The gene discussed is CARS1; the disease is Anxiety.