The cytotoxicity of SPIONs@poly(sodium styrene sulfonate)/irinotecan/human serum albumin-anti-CD133 (SPIONs@PSS/HAS-anti-CD133) were assessed in three kind of colorectal cancer cell line, including Caco2, HCT116, DLD1 cells. The gene discussed is PROM1; the disease is colorectal cancer.