Next, we performed immunohistochemistry analysis in fixed tissue biopsies from patients with metastatic malignant melanoma (TNM8 Stage 3) and non‐metastatic lymph nodes (prostate neoplasm; pN0 lymph nodes) (control) to further investigate: (i) the differential expression and distribution of α‐syn and DJ‐1 in metastatic melanoma lymph nodes compared to non‐metastatic non‐melanoma lymph nodes and (ii) whether specific pathological forms of α‐syn may be detected in melanoma lymph node metastasis. This evidence concerns the gene PARK7 and metastatic melanoma.