DN severity was considered as the dependent variable (NO=1, MI=2, MA=3), UA, SCr, TG, FINS, eGFR,GPX4, ACSL4, iron, Tf, and Ferritin as independent variables, multinomial logistic regression analysis was performed to evaluate the associations between DN severity and GPX4, ACSL4, iron, Tf, and Ferritin levels. Here, ACSL4 is linked to liver dysplastic nodule.