In the study, we found that the severity of DN was negatively correlated with serum GPX4 and positively correlated with ACSL4; however, the association between serum GPX4 and ACSL4 levels and expression levels of GPX4 and ACSL4 in renal tissue is not clear and further studies may be needed in the future. This evidence concerns the gene ACSL4 and liver dysplastic nodule.