DN severity was considered as the dependent variable (NO=1, MI=2, MA=3), UA, SCr, TG, FINS, eGFR,GPX4, ACSL4, iron, Tf, and Ferritin as independent variables, multinomial logistic regression analysis was performed to evaluate the associations between DN severity and GPX4, ACSL4, iron, Tf, and Ferritin levels. This evidence concerns the gene GPX4 and liver dysplastic nodule.