The results indicated that factors such as TNM stage (χ2 = 10.810, log-rank P = 0.001), high expression of SHMT2 (χ2 = 7.279, log-rank P = 0.007), T stage (χ2 = 6.181, log-rank P = 0.019), M stage (χ2 = 21.903, log-rank P < 0.001), pathological grade (χ2 = 5.006, log-rank P = 0.025), tumour size (χ2 = 8.876, log-rank P = 0.005), and vessel invasion (χ2 = 29.205, log-rank P < 0.001) were associated with poor prognosis (Figs. 2C–2I). This evidence concerns the gene SHMT2 and neoplasm.