We previously demonstrated that anti-CTLA-4 and/or anti-PD-1 induces macrophage TME remodeling characterized by a reduction in M2-like macrophages co-expressing the fractalkine receptor (CX3CR1) and CD206 and an increase in M1-like iNOS+ macrophages in mouse MCA sarcoma models19,21. This evidence concerns the gene NOS2 and sarcoma.