EDN1 and type 2 diabetes mellitus: In the current case-control study that included 97 subjects with T2DM, and 80 healthy individuals, the determination of rs2071942 G/A, rs5370 gene polymorphisms in EDN1 gene was conducted by gene amplification using PCR-specific primers and then genotyping through digesting the PCR products by Сас81 enzyme for rs5370 G > T and Taq1 enzyme for rs2071942 G/A.