TBX20 and atrial septal defect: For the variants with greater AF of TBX20, Santillán in 2015 [25], reports c.39T > C, c.545 + 13A > G and c.655-44G > A as gene variants associated with heart disease; c.655-44G > A was reported by Monroy et al. 2015 in patients with ASD [16]; c.381-40A > G has not been previously reported in the literature.