Here, we investigated the impact of DMT1 silencing on PINK1/Parkin-dependent mitophagy, as well as on FTMT expression, whose induction had been recently associated with iron chelation-induced mitophagy in cancer cells [37], and on the global autophagy marker LC3B-II in both MDA-MB-231 and T47D breast cancer cells. This evidence concerns the gene PRKN and cancer.