Since DMT1 is detected bridging Tf-containing EE with mitochondria in MDA-MB-231 breast cancer cells, we then evaluated the role of DMT1 in EE-mitochondria association by silencing DMT1 using CRISPR/Cas9 in MDA-MB-231 or T47D breast cancer cell lines (Fig. 1B). The gene discussed is TF; the disease is breast cancer.