This lead to the identification of five clusters reflecting different cell type mixtures; proliferative/keratin (prolif.keratin, primary melanoma), proliferative (prolif, metastatic melanoma with proliferative signature, MITF high), invasive (AXL, JUN, TFAP2B, TFAP2C high), proliferative/immune (prolif.immune, metastatic melanoma with proliferative and immune signatures) and, finally, proliferative/immune/invasive (metastatic melanoma samples with proliferative, immune and invasive gene signatures expressed). The gene discussed is TFAP2C; the disease is metastatic melanoma.