Based on the expression of SDC1, LUM, VEGFA, TIMP3 and WNT7B in various urothelial carcinoma cell lines in the CCL database, we selected J82 and UMUC3 as the verification cell lines (Additional file 1: Figure S6A), and marked them with puromycin resistance labelling. The gene discussed is VEGFA; the disease is urothelial carcinoma.