As shown in Additional file 3: Fig. S5B, culture of 3T3-L1 adipocytes in COL-I-based 3D microgels enriched in the proteoglycan, lumican (i.e., obesity conditions), induced a decrease in the number of LDs immunostained for Rab34, though no changes were observed in total Rab34 immunosignal per cell. Here, RAB34 is linked to obesity due to melanocortin 4 receptor deficiency.